Untargeted Metabolomics of the Sea Cucumber, Bohadschia marmorata (Jaeger, 1833)

  • Wilson L. Laranang Department of Laboratories, Ilocos Training and Regional Medical Center, City of San Fernando, La Union 2500 Philippines | School of Advanced Studies, Saint Louis University, Baguio City, 2600 Philippines
  • Dianne A. Peralta University Research and Extension Office, Don Mariano Marcos Memorial State University – North La Union Campus, Bacnotan, La Union 2515 Philippines
  • Victoria N. Malaya College of Fisheries, Don Mariano Marcos Memorial State University – South La Union Campus, Santo Tomas, La Union 2505 Philippines
  • Paulina A. Bawingan School of Advanced Studies, Saint Louis University, Baguio City, 2600 Philippines
Keywords: holothurians, metabolites, Philippine sea cucumber, sea cucumber spicules, triterpenoid saponin


The marine invertebrates such as sea cucumber are regarded as sources of diverse bioactive compounds with promising health and nutritional benefits. Many commercially traded sea cucumber species have been explored, yet none of the local low-value types in the province of La Union, Philippines. This study aimed to establish a baseline metabolomic profile of Bohadschia marmorata (Jaeger, 1833). The identification of compounds in its body wall utilized an Orbitrap MS-based approach. Metabolites were annotated using Compound Discover 3.2, and similarity searches were performed from online databases. Eleven putatively identified compounds, which included saponin (3beta,5xi,9xi)-28-Hydroxy-28-oxoolean-12-en-3-yl-beta-Dgalac topyranosyl-(1-3)-[beta-D-glucopyranosyl-(1-2)]-beta-D-glucopyranosiduronic acid, fatty acids and their byproducts such as eicosanoid (11,12-Epoxy-(5Z,8Z,11Z)-icosatrienoic acid) and prostanoids (13,14-dihydro Prostaglandin F1?; 13,14-Dihydro-15-keto Prostaglandin A2), dicarboxylic acids (tetradecanedioic acid; dodecanedioic acid; suberic acid; NP-001596), and amino acid (4-Oxoproline). The metabolic profile of the B. marmorata body wall showed a diverse group of representative metabolites common among holothurians associated with innate defense mechanisms, structural composition, and products of metabolic pathways. There is, however, a need to further isolate these compounds using other methods and test their biological activities.